n nervonoyl sphingosylphosphorylethanolamine (Avanti Polar)
Structured Review

N Nervonoyl Sphingosylphosphorylethanolamine, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/n nervonoyl sphingosylphosphorylethanolamine/product/Avanti Polar
Average 90 stars, based on 2 article reviews
Images
1) Product Images from "Formation of tubules and helical ribbons by ceramide phosphoethanolamine-containing membranes"
Article Title: Formation of tubules and helical ribbons by ceramide phosphoethanolamine-containing membranes
Journal: Scientific Reports
doi: 10.1038/s41598-019-42247-1
Figure Legend Snippet: Steady-state anisotropy ( r ) of DPH in various CPE-based suspensions. Lipid suspensions were prepared from pure CPEs as described in Materials and Methods. The anisotropy of DPH is drawn as a function of the temperature. C12:0, d17:1/ N -12:0 CPE; milk, milk CPE; C16:0, d18:1/ N -16:0 CPE; C18:1, N -18:1 CPE; C24:1, d18:1/ N -24:1 Δ15 (c) CPE; C24:0, d18:1/ N -24:0 CPE.
Techniques Used:
Figure Legend Snippet: Darkfield optical micrographs of aqueous dispersions of various CPE:diC18:1 PC (1:1) suspensions. ( A ) diC18:1 PC, ( B , H ) d17:1/ N -12:0 CPE:diC18:1 PC, ( C ) d18:1/ N -16:0 CPE:diC18:1 PC, ( D ) N -18:1 CPE:diC18:1 PC, ( E ) d18:1/ N -24:0 CPE:diC18:1 PC, ( F ) d18:1/ N -24:1 Δ15 (c) CPE::diC18:1 PC, ( G ) milk CPE:diC18:1 PC. Lipid suspensions were prepared in MilliQ water at ( A , G , H ) 60 °C, ( B ) 32 °C, ( C ) 61 °C, ( D ) room temperature (22 °C), ( E ) 67 °C and 41 °C ( F ) as described in Materials and Methods. Bars, 10 μm.
Techniques Used:
Figure Legend Snippet: Binding of PlyA2 to CPE:diC18:1 PC (1:1) suspensions. ( A , B ) PlyA2-EGFP was added to pre-formed milk CPE:diC18:1 PC (1:1) helices at room temperature. Darkfield ( A ) and fluorescence ( B ) images were acquired after 30 min incubation. ( C – F ) PlyA2-EGFP was added to d18:1/ N -24:1 Δ15 (c) CPE:diC18:1 PC during hydration of the lipid film at 41 °C for 3 h, followed by darkfield ( C , D ) and fluorescent ( E , F ) microscope analysis. Dotted squared area in ( C ) and ( E ) were enlarged in ( D ) and ( F ), respectively. ( G , H ) mCherry-NT-Lys was added to d18:1/ N -24:1 Δ15 (c) CPE:diC18:1 PC as described above and darkfield ( G ) and fluorescent ( H ) images were acquired. Bars, 1 μm.
Techniques Used: Binding Assay, Fluorescence, Incubation, Microscopy